In vitro culture of worms - a powerful Tool*

In vitro culture of schistosomes - from larvae to adults

The in vitro culture protocol established at helminGuard allows the stable maturation of Schistosoma mansoni larvae into adult parasites including deposition of immature eggs. The worms are kept with human blood/constituents to mimic their natural environment, i.e. human blood/vessels. Thus, immune cells and immune factors are present in the assay milieu. The presence of these factors is essential for potentiating the mode of action of anthelminthic compounds such as praziquantel, and represents a key advantage of this functional assay format over target-based biochemical or cellular assays.

In vitro-grown 18-day-old Schistosoma mansoni larvae

In vitro-matured adult S. mansoni couples surrounded by immature eggs

*supported by the Federal Ministry for Education and Research Program “Alternative Methods to Animal Experiments”, BMBF/PTJ 0315277